In June 2021 at plant growth stage 39 (BBCH scale), colleagues of the project partners TI and FAR collected samples of soil, plants and litter in all treatments. Furthermore, sampling included soil microorganisms, nematodes, soil microarthropods and earthworms. These samples are part of case study 11, Plant diversity is tested to promote soil intrinsic self-regulating processes and to enhance fungivorous soil fauna communities in wheat-cropping systems. Continental (Germany).